SIBO Lactulose Breath Test – FMD

FM Diagnostics - A NABL Approved Lab

SIBO Lactulose Breath Test


Test Parameters:

Hydrogen and methane breath testing is a widely accepted means of identifying changes to the gut microbiome and is used to detect abnormal growth of bacteria in the small intestine, or small intestinal bacterial overgrowth (SIBO).

Reporting Time: 2-4 weeks
Breath Test
Best and Honest Price

Check Your PIN Code

Pincode field should not be empty!

Check if your PIN Code is servicable. Providing PIN Code for this test is mandatory.

Product Description

Hydrogen (H2) and methane (CH4) gasses are produced in the digestive system primarily only by the bacterial fermentation of carbohydrates (sugars, starches or vegetable fibers). The generation of H2 and/or CH4 will result in the absorption of some of these gases into the bloodstream from the site of their production, and they will appear in the expired air. If either of the gases appear in the expired air, it is usually a signal that carbohydrates or carbohydrate fragments have been exposed to bacteria, permitting such fermentation to take place and that SIBO can be suspected.

Studies have indicated that potentially up to 80% of patients with IBS may in fact have SIBO which this Hydrogen /Methane breath test can easily and non-invasively help determine.

Who should do the SIBO test?

Individuals experiencing the following symptoms should consider doing a SIBO test

  • Abdominal bloating
  • Abdominal pain
  • Asthma
  • Belching
  • Bloating
  • Constipation
  • Cramping
  • Diarrhea
  • Fatigue
  • Food sensitivities
  • Gas
  • Headaches
  • Joint pain
  • Malabsorption
  • Malnutrition
  • Mood issues
  • Nausea
  • Skin issues
  • Weight loss
Specimen : Breath


  • Vacuum-sealed collection tubes

Patient preparation

  • Requires fasting 12 hours prior to collection
  • This test will take 3 hours to complete
  • Preparation for the test starts 2-4 weeks before collection ( Please check sample collection instructions)

* Please note this test will take 3 hours to complete.


  • Bures J, Cyrany J, Kohoutova D, et al. Small intestinal bacterial overgrowth syndrome. 2010;16(24).
  • Chedid V, Dhalla S, Clarke JO, et al. Herbal therapy is equivalent to Rifaximin for the treatment of small intestinal bacterial Overgrowth. Global Advances in Health and Medicine. 2014;3(3):16–24.
  • Dukowicz AC, Lacy BE, Levine GM. Small intestinal bacterial Overgrowth. 2007;3(2).
  • Erdogan A, Rao SSC. Small Intestinal Fungal Overgrowth. Current Gastroenterology Reports. 2015;17(4).
  • Fasano A. Leaky gut and autoimmune diseases. Clinical reviews in allergy & immunology. 2011;42(1):71–8.
  • Gabbard S, Lacy B, Levine G, Crowell M. The impact of alcohol consumption and cholecystectomy on small intestinal bacterial overgrowth. Digestive diseases and sciences. 2013;59(3):638–44.
  • Ghoshal UC, Srivastava D, Ghoshal U, Misra A. Breath tests in the diagnosis of small intestinal bacterial overgrowth in patients with irritable bowel syndrome in comparison with quantitative upper gut aspirate culture. European Journal of Gastroenterology & Hepatology. 2014;26(7):753-760.
  • Ghoshal UC. How to Interpret Hydrogen Breath Tests. Journal of Neurogastroenterology and Motility. 2011;17(3):312-317.
  • Hauge T, Persson J, Danielsson D. Mucosal bacterial growth in the upper gastrointestinal tract in alcoholics (heavy drinkers). Digestion. 2009;58(6):591–595.
  • Lauritano E, Gabrielli M, Scarpellini E, et al. Small intestinal bacterial overgrowth recurrence after antibiotic therapy. The American journal of gastroenterology. 2008;103(8):2031–5.
  • Levitt MD. Volume and Composition of Human Intestinal Gas Determined by Means of an Intestinal Washout Technic. New England Journal of Medicine. 1971;284(25):1394-1398.
  • Lloyd-Still JD, Shwachman H. Duodenal microflora. The American Journal of Digestive Diseases. 1975;20(8):708-715.
  • Methodology and Indications of H2-Breath Testing in Gastrointestinal Diseases: the Rome Consensus Conference. Alimentary Pharmacology & Therapeutics. 2009;29:1-49.
  • Newberry C, Tierney A, Pickett-Blakely O. Lactulose Hydrogen Breath Test Result Is Associated with Age and Gender. BioMed Research International. 2016;2016:1-5.
  • Rana S, Sharma S, Kaur J, Sinha S, Singh K. Comparison of Lactulose and Glucose Breath Test for Diagnosis of Small Intestinal Bacterial Overgrowth in Patients with Irritable Bowel Syndrome. Digestion. 2012;85(3):243-247.
  • Rana SV, Bhardwaj SB. Small intestinal bacterial overgrowth. Scandinavian Journal of Gastroenterology. 2008;43(9):1030-1037.
  • Roland B, Ciarleglio M, Clarke J, et al. Small intestinal transit time is delayed in small intestinal bacterial Overgrowth. Journal of clinical gastroenterology. 2014;49(7):571–6.
  • Saad RJ, Chey WD. Breath Testing for Small Intestinal Bacterial Overgrowth: Maximizing Test Accuracy. Clinical Gastroenterology and Hepatology. 2014;12(12):1964-1972.
  • Shah ED, Basseri RJ, Chong K, Pimentel M. Abnormal Breath Testing in IBS: A Meta-Analysis. Digestive Diseases and Sciences. 2010;55(9):2441-2449.

SIBO Lactulose Breath Test

The best way to determine the presence of SIBO is to perform a hydrogen breath test. This simple procedure requires administering a challenge substrate (either lactulose or glucose), collecting patients’ breath samples with an easy to use straw and tube method, and sending the samples to our lab for measurement of hydrogen and methane levels. Test kits can be used in your office or sent home with your patient. A prepaid mailer to return the breath samples to the lab comes with each kit. Upon arrival, tests are analyzed and a report faxed or emailed to you within 24 hours of test receipt.

Order an SIBO Lactulose BreathTest it is just a few simple step!


please enter the data where the SIBO Lactulose Breath set should be send


Detailed instructions and a return envelope you will find in the SIBO Lactulose Breath set


After 2-4 weeks, you will receive detailed SIBO Lactulose Breath analysis.

Our Working Process Healthcare model is shifting from Sickness care to Wellness.

Sample Collection

Sample collection will be done from home

Sample Processing

Sample is processed at Central Lab of our Trusted Partners.

Online Reporting

Reports are accessible online.

Sample Collection

Sample collection will be done from home

Sample Processing

Sample is processed at Central Lab of our Trusted Partners.

Online Reporting

Reports are accessible online.

Autism Test FAQ's Functional Medicine Diagnostic FAQ's

Autism is a systemic body disorder that affects the brain.
Earlier the Medical community believed that autism was caused by “bad mothering” or the genes caused it. Today, there is enough scientific evidence that Autism is a whole body disorder triggered by a toxic environment, inflammatory food, blue light toxicity, infections, etc.
Several studies suggest autism is a whole body disorder. Besides impacting the social and cognitive abilities of the child, it is observed that affected individuals are also sensitive to food and environmental toxins due to poor linings in stomach and brain which results in full body symptoms like itching and dry skin, head sweats, bloating and upset stomach, urinary hesitancy, walking on toes, head banging, etc.
Autism is a spectrum disorder hence, symptoms can vary in severity and type from person to person. Most commonly seen symptoms of ASD are:
  • Difficulty with communication which could be inability to speak or delayed speech
  • Inappropriate use of gestures, eye contact, facial expressions, tone of voice
  • Difficulty recognizing, expressing and responding to emotions
  • Feeling uncomfortable in social situations
  • Extreme interest or non-interest in specific topics
  • Repetitive body movements (rocking, flapping hands or spinning)
  • Resistance to change
  • Specific food preferences or aversions
  • Sensory Processing differences
  • Immune system abnormalities
  • Gastrointestinal symptoms (constipation, loose stools, frequent stomach aches)
  • Lining up objects
  • Labile mood, increased tantrums or emotional outbursts
Several factors can increase the likelihood of a child suffering from Autism. The most common ones are:
  1. Gut dysbiosis: Children who have gastrointestinal issues have been seen to have a less diverse gut microbiome. The imbalance between the good and bad bacteria in the gut creates toxins that directly affect the brain.
  2. Toxins: Autistic children are more likely to accumulate harmful environmental toxins like lead, mercury, aluminium etc in their bodies, which can in turn cause their mitochondria to be less efficient in producing energy.
  3. Nutritional Deficiencies: Tendency of kids to have a narrow range of food leads them to nutrient deficient states. Deficiencies in nutrients needed for brain health like B vitamins, Omega 3 fats, zinc, magnesium and selenium are common.
  4. Food Sensitivities: Food sensitivities are often caused by the digestive lining becoming too leaky, allowing toxins, bacteria and incompletely digested particles of food into the bloodstream. A leaky gut also encourages autoimmune issues. It’s believed an attack by the immune system on brain cells, causing inflammation, could be a factor in some cases of autism.
  5. Oxidative Stress: Mitochondria is the powerhouse of energy. Brain requires a lot of energy for proper function. Mitochondrial dysfunction is commonly seen in autistic children.
There is an existence of a gut–brain axis, in which gut microbes produce bioactive compounds that influence brain function. Several researches have bolstered this theory, showing that when gut bacteria help to digest food, they generate a host of by-products that can affect thinking and behaviour. Researchers have also observed that some people with ASD could have an abnormally porous blood–brain barrier, which allows some toxic bacterial by-products to enter the bloodstream and reach the brain.
Parents play a central role in the intervention for Autism and Home is the prime centre for treating autism. The interventions that can be followed up by parents are:
  1. Diet: Parents should implement an autism diet, autism-specific nutrition, and specialized supplementation. Most parents begin with the GF/CF diet as children on the spectrum are usually highly allergic (or sensitive) to gluten and casein, the protein found in wheat, flour, and milk.
  2. Grounding: The simplest way of grounding involves walking or running barefoot outside on grass, sand or soil. Moist ground or grass is the perfect electrical conductor. Sitting Down Barefoot with your feet on grass or soil and avoid shoes with plastic and other soles are other ways of staying connected with earth.
  3. Monitoring screen time: Research shows that excess screen time results in over stimulation of the frontal lobe and an antagonist under development of the language centre in the brain. Limiting screen time also decreases exposure to toxic blue light which has an impact on gut microbiome.
Autism susceptibility is currently estimated to be 40–80% genetic. Environmental factors – likely acting through epigenetic regulation as the major mechanism – presumably compromise the remainder of the risk. Hundreds of potential environmental factors have been suggested to contribute to risk, such as increased parental age (especially paternal), maternal complications or infections during pregnancy. It has been said that genetics loads the gun, but environment is what pulls the trigger to cause autism in the majority of children.

There are historical differences between the term “Asperger’s” and what’s considered “autism.” Asperger’s was first introduced into the Diagnostic and Statistical Manual of Mental Disorders (DSM) in 1994, and was considered as a “mild” or “high-functioning” form of autism.

This happened because English psychiatrist Lorna Wing translated the works of Austrian physician Hans Asperger and realized his research found distinct characteristics in autistic children from those with “milder” symptoms. However, as of 2013, Asperger’s is now considered part of the autism spectrum and is no longer diagnosed as a separate condition. The only real “difference” between the two diagnoses is that people with Asperger’s may be considered as having an easier time “passing” as neurotypical with only “mild” signs and symptoms that may resemble those of autism.

Conventional diagnosis involves finding out whether the child’s behaviour meets a set of criteria for autism or ADHD, based on a set of questionnaires.

Functional Medicine approach is to individualize each and every autistic child and find the root cause of the imbalance. Tests commonly advised are to assess gut microbiome. Functional testing can also look at toxic load, nutritional status, food sensitivities, mitochondrial function and genetic susceptibility to poor detoxification.

Functional medicine approaches Autism as a whole body disorder and blood tests help in understanding the overall functioning of the entire body.
According to the Autism Society of America, “as there is no one symptom or behavior that identifies individuals with ASD, there is no single treatment that will be effective for all people on the spectrum. Individuals can learn to function within the confines of ASD and use the positive aspects of their condition to their benefit, but treatment must begin as early as possible and be tailored to the child’s unique strengths, weaknesses and needs.’’
Researchers say the chance of having autism spectrum disorder (ASD) appears to increase with the age of the parents when a child is born. It is believed that children born to older parents have a 50% higher chance of ASD.
Monitoring, screening, evaluating, and diagnosing children with ASD as early as possible is important to make sure children receive the services and support they need to reach their full potential. Many children show symptoms of autism by 12 months to 18 months of age or earlier, like poor eye contact or non responsiveness to their own name.

Other Popular Test Packages Choose from our frequently booked test packages.